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Abstract
The current study was carried out to isolate Actinomycetes bacteria from soil samples from AlDiwaniyah City, Iraq, and to evaluate the toxicity and antioxidant characteristics of the extract of the isolated species. For that aim, 500gm of soil sample was collected and subjected to series of bacterial cultivation that ended up with bacterial identification using morphological, biochemical, and 16S rRNAgene-polymerase chain reaction (PCR) tools. The final identification revealed the isolation of two Actinomycetes; Streptomyces sp. (named as QZS11) and Nocardia sp. (named as QZS9), and since many members of Streptomyces are beneficial bacteria, the toxicity and antioxidant characteristics of the extract of the isolated QZS11 were evaluated using the Vero cell viability assay and the diphenyl picryl hydrazine (DPPH) reduction assay, respectively. The results showed that the viability rates of the Vero cells were 100% at 0.125mg/ml and 90% at 0.25mg/ml. The findings regarding the DPPH assay revealed an important antioxidant level (20%) at 0.25mg/ml. The study reports the isolation of two Actinomycetes; Streptomyces sp. (QZS11) and Nocardia sp. (QZS9), with low toxicity and important antioxidant level at a very low concentration for the QZS11 isolate.
Keywords
Actinomycetes
antibiotics
Nocardia
Streptomyces
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This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
Asseel Abdulridha Saeed, & Ishtar Adnan Mohamed Alethari. (2023). Molecular identification of two Actinomycetes (Streptomyces sp. and Norcardia sp.) with the evaluation of cytotoxicity and antioxidant properties of Streptomyces sp. Extract. Texas Journal of Agriculture and Biological Sciences, 12, 49–56. Retrieved from https://zienjournals.com/index.php/tjabs/article/view/3262
References
- Bérdy J. Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot (Tokyo) [Internet]. 2012 [cited 2022 Mar 1];65(8):385–95. Available from: https://pubmed.ncbi.nlm.nih.gov/22511224/
- Newman DJ, Cragg GM. Natural Products as Sources of New Drugs from 1981 to 2014. J Nat Prod [Internet]. 2016 Mar 25 [cited 2022 Mar 1];79(3):629–61. Available from: https://pubmed.ncbi.nlm.nih.gov/26852623/
- Morgan S, Grootendorst P, Lexchin J, Cunningham C, Greyson D. The cost of drug development: a systematic review. Health Policy [Internet]. 2011 Apr [cited 2022 Mar 1];100(1):4–17. Available from: https://pubmed.ncbi.nlm.nih.gov/21256615/
- DiMasi JA, Grabowski HG, Hansen RW. Innovation in the pharmaceutical industry: New estimates of R&D costs. J Health Econ [Internet]. 2016 May 1 [cited 2022 Mar 1];47(5):20–33. Available from: https://pubmed.ncbi.nlm.nih.gov/26928437/
- Van Norman GA. Drugs, Devices, and the FDA: Part 1: An Overview of Approval Processes for Drugs. JACC Basic to Transl Sci [Internet]. 2016 Apr 1 [cited 2022 Mar 1];1(3):170–9. Available from: https://www.jacc.org/doi/10.1016/j.jacbts.2016.03.002
- Zumla A, Petersen E. The historic and unprecedented United Nations General Assembly High Level Meeting on Tuberculosis (UNGA-HLM-TB)—‘United to End TB: An Urgent Global Response to a Global Epidemic.’ Int J Infect Dis [Internet]. 2018 Oct 1 [cited 2022 Mar 1];75(9):118–20. Available from: http://www.ijidonline.com/article/S1201971218345351/fulltext
- Mendelson M, Matsoso MP. The World Health Organization Global Action Plan for antimicrobial resistance. SAMJ South African Med J [Internet]. 2015 [cited 2022 Mar 1];105(5):325–325. Available from: http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0256- 95742015000500001&lng=en&nrm=iso&tlng=es
- Barka EA, Vatsa P, Sanchez L, Gaveau-Vaillant N, Jacquard C, Klenk H-P, et al. Taxonomy, Physiology, and Natural Products of Actinobacteria. Microbiol Mol Biol Rev [Internet]. 2015 Mar [cited 2022 Mar 1];80(1):1–43. Available from: https://pubmed.ncbi.nlm.nih.gov/26609051/
- Takahashi Y, Nakashima T. Actinomycetes, an Inexhaustible Source of Naturally Occurring Antibiotics. Antibiotics [Internet]. 2018 Jun 1 [cited 2022 Mar 1];7(2):45–70. Available from: /labs/pmc/articles/PMC6022875/
- Pitcher DG, Saunders NA, Owen RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol [Internet]. 1989 Apr 1 [cited 2022 Mar 1];8(4):151–6. Available from: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1472- 765X.1989.tb00262.x
- Lane DJ. (1991) 16S/23S rRNA Sequencing. In Stackebrandt, E. and Goodfellow, M., Eds., Nucleic Acid Techniques in Bacterial Systematic. In John Wiley and Sons, New York-Scientific Research Publishing; 1991 [cited 2022 Mar 1]. p. 115–75. Available from: https://www.scirp.org/(S(lz5mqp453edsnp55rrgjct55))/reference/ReferencesPapers.aspx? ReferenceID=1870033
- Raheel R, Ashraf M, Ejaz S, Javeed A, Altaf I. Assessment of the cytotoxic and anti-viral potential of aqueous extracts from different parts of Acacia nilotica (Linn) Delile against Peste des petits ruminants virus. Environ Toxicol Pharmacol [Internet]. 2013 Jan [cited 2022 Mar 1];35(1):72–81. Available from: https://pubmed.ncbi.nlm.nih.gov/23262040/
- Patel RM, Patel NJ. In vitro antioxidant activity of coumarin compounds by DPPH, Super oxide and nitric oxide free radical scavenging methods . J Adv Pharm Educ Res [Internet]. 2011 [cited 2022 Mar 2];1(3):52–68. Available from: https://japer.in/article/in-vitro- antioxidant-activity-of-coumarin-compounds-by-dpph-super-oxide-and-nitric-oxide-free-radical-scavenging-methods
- Sapkota A, Thapa A, Budhathoki A, Sainju M, Shrestha P, Aryal S. Isolation, Characterization, and Screening of Antimicrobial-Producing Actinomycetes from Soil Samples. Int J Microbiol [Internet]. 2020 [cited 2022 Mar 2];2020(3):2716584–90. Available from: /labs/pmc/articles/PMC7139855/
- Singh V, Haque S, Singh H, Verma J, Vibha K, Singh R, et al. Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications. Front Microbiol [Internet]. 2016 [cited 2022 Mar 2];7(12):1921–9. Available from: /labs/pmc/articles/PMC5138215/
- Shrestha B, Nath DK, Maharjan A, Poudel A, Pradhan RN, Aryal S. Isolation and Characterization of Potential Antibiotic-Producing Actinomycetes from Water and Soil Sediments of Different Regions of Nepal. Int J Microbiol [Internet]. 2021 [cited 2022 Mar 3];2021(3):5586165–73. Available from: /labs/pmc/articles/PMC7946463/
- Sudha S, Masilamani SM. Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4. Asian Pac J Trop Biomed [Internet]. 2012 [cited 2022 Mar 3];2(10):775. Available from:
- /labs/pmc/articles/PMC3609218/
- Tan LTH, Chan KG, Khan TM, Bukhari SI, Saokaew S, Duangjai A, et al. Streptomyces sp. MUM212 as a source of antioxidants with radical scavenging and metal chelating properties. Front Pharmacol [Internet]. 2017 May 17 [cited 2022 Mar 3];8(5):276–93. Available from: /labs/pmc/articles/PMC5434116/