Development Of A Complex Biotechnological System For In Vitro Propagation Of Plumeria Plants
DOI:
https://doi.org/10.62480/tjabs.2026.vol50.pp5-8Keywords:
Plumeria, in vitro propagation, biotechnology system, micropropagationAbstract
Plumeria species are highly valued ornamental and medicinal plants, but their conventional propagation methods are slow and inefficient for large-scale production. This study focuses on the development of a complex biotechnological system for the in vitro propagation of Plumeria plants. The system integrates optimized surface sterilization procedures, nutrient media composition, and plant growth regulators to ensure high regeneration efficiency. Shoot induction, multiplication, rooting, and acclimatization stages were systematically optimized under controlled laboratory conditions. The results demonstrate that the use of Murashige and Skoog (MS) medium supplemented with appropriate concentrations of cytokinins and auxins significantly enhances micropropagation efficiency. The developed protocol ensures rapid, uniform, and disease-free plant production, offering a reliable biotechnological approach for the conservation and mass propagation of Plumeria species
References
1. Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco
tissue cultures. Physiologia Plantarum, 15(3), 473–497.
2. George, E. F., Hall, M. A., & De Klerk, G. J. (2008). Plant Propagation by Tissue Culture (3rd ed.).
Springer.
3. Loyola-Vargas, V. M., & Ochoa-Alejo, N. (2018). Plant Cell Culture Protocols. Springer Protocols.
4. Thorpe, T. A. (2007). History of plant tissue culture. Molecular Biotechnology, 37(2), 169–180.
5. Rout, G. R., & Jain, S. M. (2004). Micropropagation of ornamental plants: A review. Horticultural
Reviews, 30, 415–487.
6. Razdan, M. K. (2003). Introduction to Plant Tissue Culture. Science Publishers.
7. Sharma, K. K., & Srivastava, S. (2013). Plant tissue culture: A biotechnological tool for solving
horticultural problems. Journal of Plant Science, 8(2), 45–60.
8. Kumar, N., & Reddy, M. P. (2011). Plant regeneration in ornamental species through in vitro
techniques. Plant Cell Biotechnology and Molecular Biology, 12(1–2), 1–12.
9. Debnath, M. (2005). Clonal propagation of medicinal plants through tissue culture. Plant Tissue
Culture and Biotechnology, 15(2), 105–112.
10. Pierik, R. L. M. (1997). In Vitro Culture of Higher Plants. Springer.
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