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Abstract

Although the idea of micro–organism such as bacteria in standard is dangerous to humans, A quantity of its species are taken into consideration to be of superb cost and safety, especially in organic therapy, including the institution Bacteria Probiotics. Lactobacillus salivarius is one of the maximum fundamental forms of bacteria which can be innocent to people and which may also play a main position in their bioremediation potential in general. Moreover, Lactobacillus salivarius was identified by traditional methods using MRS agar selective medium, then by biochemical methods using the VITEK 2 method. Finally, Lactobacillus salivarius was confirmed using Multi Sequencing (MLST) and Biofilm technique. The main objective of this research is to isolate and characterize the novel bacterium salivarius Lacto from Greek yogurt and their ability for biological assay and for study to determine the effect of bacteriocin, which is secreted by these bacteria, and to study the extent to which these by-products to reduce environmental pollution by removing heavy metals from various environmental media by these products.

Keywords

Lactobacillus Yogurt MLST Bio-film technique

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How to Cite
Arqam Alomari, Farah, J. A. Alameen, & Ayman Albanna. (2022). Multi-locus sequences Technique for Identified Lactobacillus salivarius from Greek yogurt anti-biofilm agents. Texas Journal of Agriculture and Biological Sciences, 10, 95–103. Retrieved from https://zienjournals.com/index.php/tjabs/article/view/2823

References

  1. Abbas, Hussein Mahmoud, and Ahmed, Mohamed Fakhri (2014). The effect of some amino acids on Biofilm of aureur staphylococcuy bacteria. Diyala Journal of Science
  2. Abdel Nasser, Iman Mohammed (2010).
  3. Abed, T.A., (2013). Evaluation of methods for the extraction and purification of DNA of cultured Lctobacillus colony isolated from dairy products. International Journal of Applied Microbiology and Biotechnology Research,1,20-25.
  4. Ahmed , T. and Kanwal. R.(2004). Biochemical Characteristics of Lactic acid Producing Bacteria and Preparation of Camel Milk Cheese by Using Starter Culture. Pakistan Vet. J .,24 (2) .
  5. Albanna, A. M. J. (2017). Regulation of flagellar mediated motility in the species Samonella enterica (Doctoral dissertation, Newcastle University).
  6. Alomari, A. (2017). Biophysical and Kinetic Analysis of Escherichia coli DNA Ligase Activity and Inhibition. Doctoral dissertation, University of Portsmouth, UK.
  7. Al-Khafaji, Zahra Mahmoud (1990). biotechnology . Ministry of Higher Education and Scientific Research / University of Baghdad. Dar Al-Hekma Press for Printing and Publishing.
  8. Allameh, S.K., Daud, H., Yusoff, F.M., Saad, C.R. and Ideris, A. (2012). Isolation, identification and characterization of Leuconostoc mesenteroides as a new probiotic from intestine of snakehead fish (Channastriatus). African Journal of Biotechnology ,11(16), 3810- 3816.
  9. Anon. (2011). Fermented Milk Products Canadian dairy commission. http://www.milkingredients.ca/index-eng.php.
  10. Bin Masalam, M. S., Bahieldin, A., Alharbi, M. G., Al-Masaudi, S., Al-Jaouni, S. K., Harakeh, S. M., Medicine, A. (2018). Isolation, molecular characterization and probiotic potential of lactic acid bacteria in Saudi raw and fermented milk. 2018.
  11. Bin Masalam, M. S., Bahieldin, A., Alharbi, M. G., Al-Masaudi, S., Al- Jaouni, S. K., Harakeh, S. M., Medicine, A. (2018). Isolation, molecular characterization and probiotic potential of lactic acid bacteria in Saudi raw and fermented milk. 2018.
  12. Corsetti, A., Lavermicocca, P., Morea, M., Baruzzi, F., Tosti, N., & Gobbetti, M. (2001). Phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species Triticum durum and Triticum aestivum) sourdoughs of Southern Italy. International journal of food microbiology, 64(1-2), 95-104.
  13. Erdogrul ,O. and Erbilir, F. (2006). Isolation and characterization of Lactbacillus bulgaricus and Lactobacillus casei from Various foods. Turk J. Biol. 30. 39-44 .
  14. Giraffa,G.,Chanishvili,N.,and widyastuti,Y.(2010).Importance of lactobacilli in food and feed biotechnology.Res. Microbiol.161,480-487. doi;10.1016/j.resmic.2010.03.001.
  15. Mathur, T., Singhal, S., Khan, S., Upadhyay, D. J., Fatma, T., and Rattan, A. (2006). Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods. Indian.J.Med.microbiol, 24(1): 25-29.
  16. Nair, P. S. and Surendran, P. K. (2005). Biochemical Characterization of Lactic Acid Bacteria Isolated From Fish and Prawn. Journal of Culture Collections. Volume 4: 48-52.
  17. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K., and Komagata, K. (1997). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacilluzs . Inter.J.Syst.Bacteriol, 47(2): 289-298.
  18. Sieladie, D.V., Zambou, N.F., Kaktcham, P.M., Cresci, A. and Fonteh, F. (2011). Probiotic properties of Lactobacilli strains isolated from raw cow milk in the western highlands of Cameroon. Innovative Romanian Food Biotechnology, 9(12), 12-28.
  19. Tamang ,J.P.,watanabe,K.,and Holzapfel,W.H.(2015).Review;diversity of microorganisms in global fermented foods and beverages.Front.Microbiol.7:377.doi:103389/fmicb.2016.00377.