##plugins.themes.academic_pro.article.main##
Abstract
The results of using isolation from the roots of pepper plants revealed symptoms of disease such as browning of the roots and rotting of a portion or the complete root caused by Fusarium solani fungus, as well as from all fields within the city of Babylon. The results of testing the effect of isolation on radish seeds revealed that the entire isolations testing of F. solani fungi resulted in a momentous reduction in the germination percentage under the probability level of 0.05, which ranged between 0% - 37% compared to the comparison treatment (with no pathogenic fungi), which was 100%. The findings showed that P. fluorecsens bacteria had a high inhibitory efficiency against an isolation of the pathogenic fungus F.solani (F.s2, Fs3 and Fs4) at a concentration of 5 × 107 (colony formation unit/ml), where the inhibition percentage was 82%, 67.77% and 75.22%, respectively compared to the comparison treatment in which the inhibition rate was 0. Beltanol pesticide achieved an inhibition rate of 100% with all isolations on PDA culture media. Also, all the treatments used, which include Pseudomonas fluorescen, Penicillium corylyphilium and salicylic acid, gave the best results in decreasing the infection with F. solani and with significant differences between them and the fungus treatment alone, where the treatment of bacteria fluorescens.P + P. corylyphilium, the infection severity of the fungus F. solani was less, as it reached 5% in comparison to the treatment of the pathogenic fungus alone, by 80%. As for the treatment of the bacteria fluorescens.P + p.corylyphilium together with the presence of the pathogenic fungus and in the absence of the pathogenic fungus, the highest value in length and fresh and dry weight of the vegetative and root groups, reached 28.50 and 48.50 cm, 52.50, 7.07, 44.00, and 5.03 g, correspondingly, in comparison to the treatment of pathogenic fungi alone. The average length and fresh and dry weight of the vegetative and root groups reached 18.75 and 26.00 cm 15.5, 2.26, 7.00 and 0.19 g respectively.
Keywords
##plugins.themes.academic_pro.article.details##
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
- Tewari, J.P. 2001. Guide to Commercial Greenhouse Sweet Bell Pepper Production in Alberta, Government of Alberta: .Agriculture and Rural Development.
- Kurt.S.Aysun.U.E.mine.K.merve.and Soner.S.2020. Characterization and pathogenicity of Fusarium solani associated with dry root rot of citrus in the eastern Mediterranean region of Turkey .Journal of General plant pathology. 86(4).
- McGovern, R.J., W.H. Elmer , D.M. Geiser and B.K. Harbaugh. 2002. Biology, Epidemiology and Integrated Management of Disease Caused by Fusarium in Potted Orinamental Progress Reports. http://endowment. Org / archives / 2002 / 06 / biolog - epidemiology - and - integrated.
- Sageera.S,Imtiyaz.M,Omi.L.and Arif.A.2012.Biological Control of Fusarium Oxysporum and Aspergillus Sp.By Pseudomonas Fluorescens Isolated From Wheat Rhizosphere Soil Of Kashmir. ISSN : 2278-3008 Volume 1, Issue 4 (July-August 2012), PP 24-32
- Tajick, M. A. ; M. Khani, H.S. and Babaeizad, V.2014. Identification of Biological Secondary Metabolites in Three Penicillium Species, P. Goditanum, P. Moldavicum, and P. Corylophilum. Vol. 4, N0.1, 53-61.
- Booth, C. 1977. Fusarium. Laboratory Guide to the Identification of the Major Species . England:Commonwealth Mycological Institute, Kew, Survey, 58 pp.
- Leslie, J. F. and B. A . Summerell. 2006 . The FusariumLaboratory Manual. 388.pp.
- Bolkan, H. H. and E. E. Butler .1974. Studies on Heterokaryosisvirulenc Rizoctonia solani. Phytopathology. 64: 513-522.
- PaultizT.C,T.ZhouandL.Rankin1992.Selectionof Rhizosphere Bacteria for Biologicalcorolofpythiumaphanidermatumonhydroponicallygrowncucumber.Biological control 2:22-237.
- Clark, F. E ; 1965 . Agar–plats Method for Total Microbial Count. C. F: Black, 1965. Method of Soil Analysis Part 2, U.S. A: Publisher Madison Wisconson,. pp. 1572.
- Bell, D. K.; H. D. Well, and G. R. Markham . 1982 . Invitro Antagonism of Trichoderma Spp. against Six Fungi, Plant Pathogens. Phytopathology. 72: 379-382.
- Mckinney, H.H. 1923. Influence of Soil Temperature and Moisture on Infection of Wheat Seedling by Helminthosporum Sativum. J. Agric. Research 26: 195–217.
- Al-Rawi, Kh. M. and A. M. Khalaf-Allah. 2000. Design and Analysis of Agricultural Experiments.. 2ndEdition . Iraq: Faculty of Agriculture, University of Al Mosul.
- Al-Jubouri, H. H. Sh., 2002. Effect of Using a Growth Retardant Cultar and Some Plant Extracts on the Infection of Bean Plants with Root Rot Pathogens. Master's Thesis - College of Agriculture - University of Baghdad.
- Aboud , H.M. Said, S.A.; Saleh, H.M. and Diwan, H.M. 2001. Evaluation studies of four isolate of Thielavopsis paradaxa. The Scientific Journal of Iraq Atomic Energy commission, 3: 150-155.
- -Blanco, J., Vander Drift, K. M., Thomas – Oates, J. E., Van loon, L. C. and Bakker, P. A. H. (2001). Lnfluence of growth conditions on the production of salicylic acid and psedom-onine in Pseudomonas fluorescens. phytopathology
- Fox, E.M. and B.J.Howlett. 2008. Secondary Metabolism: Regulation and Role in Fungal Biology. Curr. Open. Microbiol., 11, 481-487.
- Yin, W. and N.P.Keller. 2011. Transcriptional Regulatory Elements in Fungal Secondary Metabolism. J. Microbiol., 49, 329-339.
- Domenech, J.; Reddy, M.S.; Klopper, J.W.; Ramos, B. and J. Gutierrez-Manero. 2006. Combined Application of the Biological Product LS213 with Bacillus, Pseudomonas or Chrysebacterium for Growth Promotion and Biological Control of Soil-borne Disease in Pepper and Tomato. Biocontrol.51:245-258.
- Morsy.E.M;Abdel-Kawi.K.A.andKhalil.M.N.2009.Efficiency of Trichoderma viride and Bacillus subtilis as Biocontrol agents gainst Fusarium solani on tomato plants .pp.47-57.
- Ryals, E. Ward, S. Uknes and J. Herzog. 1994. Induction of Systemic Acquired Disease Resistance in Plants by Chemicals. Ann. Rev. Phytopathology. 32 : 439-459.
- Siegrist, J. , W. Jeblick and H. Kauss. 1994. Defense reponses in infected and elicited cucumber hypocotyl segments exhibiting acquired resistance. Plant Physiology. 105(4): 1365-1374.
- Meister, R. T. 2000. Farm Chemical Handbook. Listing for “Beltanol” Willough by OH. Vol. 86. p.45.
- Ghini, R., Mezzalama, M., Ambrosoli, R., Barberis, E., Gari- bald bald, A. and Piedade, S.M. 2000. Fusarium Oxysporum Strains as Biocontrol Agents against Fusarium wilt: Effects on soilmicrobial biomass and Activity. Brasil: Pesq.Agropec. Brass. ,Br., 35:93-101.
- Larena, I., P. Melgarejo and A. DeCal, 2002. Production, Survival, and Evaluation of Solid-substrate Inocula of Penicilliumoxalicum Biocontrol Agent against Fusarium wilt of Tomato. Phytopathology 92:863-869.
- Schisler, D.A.; N.I., Khan and P.J.Slininger.2002. Greenhouse and Field Evaluation of Biological Control of Fusarium Head Blight on DurumWheat .Plant Dis.86:1350-1356.