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Abstract
The study aimed to investigate the bioactivity effect of different concentration of ethanol Iraqi propolis against two species of bacteria. Staph.aureus and pseudomonas that were infected wound in skin of mice ,Results revealed that the concentration of 4000 and 5000 µg/ml of Iraqi propolis extracted by 75% ethanol was the most effective in growth inhibition of Staph.aureus and pseudomonas in agar diffusion method and the diameter of inhibition in Staph.aureus reached (22.30, 22.50), mm. respectively, while the diameter of inhibition of pseudomonas in a concentration of 4000 and 5000 µg/ml was (21.12,23 .60) mm. by the agar diffusion method as shown propolis was highly effective against Staph.aureus and pseudomonas in agar diffusion method with comparing the antibiotic Amoxicillin 30µg with different concentration of ethanol extract of the Iraqi propolis showed no inhibition of the Ampicillin30µg, Methoprim 25 and gentamycin 100 µg against Staph.aureus and pseudomonas. The previous concentration (4000,5000) was also superior in comparing with Ordinary ointment in terms of its effect on the healing of contaminated and non-contaminated wounds, as well as the return of the tissue to its normal position in a shorter time. where The propolis extract showed a high potency in treating wounds infected with Gramnegative (pseudomonas) and Gram-positive bacteria(Staph.aureus).
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References
- Adham, Eithar., Hassan Amal., Evaluating the role of propolis and bee venom on the oxidative stress. Scientif reports., 2022, 02-16,
- Alencar , S. M .;Oldoni, T.L. ,Castro, M .L. ;Carbal , I.. S. ; Costa-Neto C. M. ,Cury J.A. ;Rosalen P.L. ;Ikegakim , M.(2007) Chemical composition and biological activity of new type of Brazilian propolis:Red propolis Ethnopharmacol,5: 113(2):278-283.
- Al-Musafer, Mohsen Abdullah Karim (2005). The biological effectiveness of propolis, royal jelly, and bee venom on European foulbrood bacteria. Master's thesis - Technical College – Musayyib.
- Al-Waili N. S. and K. Saloom Y., “Effects of topical honey on post-operative wound infections due to gram positive and gram negative bacteria following caesarean sections and hysterectomies,” European Journal of Medical Research, vol. 4, no. 3, pp. 126–130, 1999.View at: Google Scholar
- Boukraa L., Benbarek H., and Moussa A., “Synergistic action of starch and honey against Candida albicans in correlation with diastase number,” Brazilian Journal of Microbiology, vol. 39, no. 1, pp. 40–43, 2008.View at: Publisher Site | Google Scholar.
- Dustmann J. H., “Antibacterial effect of honey,” Apiacta, vol. 14, no. 1, pp. 7–11, 1989.View at: Google Scholar.
- Elisabetsky E., Balick M. J., and Laird S. A., Medicinal Resources of the Tropical Forest: Biodiversity and Its Importance to Human Health, Columbia University Press, New York, NY, USA, 1996.
- Gulluce M., Sokmen M., Daferera D. et al., “In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L,” Journal of Agricultural and Food Chemistry, vol. 51, no. 14, pp. 3958–3965, 2003.View at: Publisher Site | Google Scholar
- Hammer K. A., C. F. Carson, and Riley T. V., “Antimicrobial activity of essential oils and other plant extracts,” Journal of Applied Microbiology, vol. 86, no. 6, pp. 985–990, 1999.View at: Publisher Site | Google Scholar.
- Hannan A., Barkaat M., Saleem S., Usman M., and Gilani W. A., Manuka honey and its antimicrobial potential against multi drug resistant strains of Typhoidal salmonellae, Ph.D. thesis, Department of Microbiology, University of Health Science, Lahore, Pakistan, 2004.
- Hegazi, A.G, and Abd El Hady, F.K. (2002B) Egyptian Propolis: antioxidant, antimicrobial activities and chemical composition of propolis Naturforsch.57: 395-402.
- Krell. R. (1996), value-Added products from bee keeping- food and Agriculture organization of the United Nations (FAO)-Roma. 157-193.
- Kumazawa ,S.:Hamasaka,T. and Nakayama T.(2004) antioxidant activity of propolis of various geographic origions,Food Chem.,84:329-339.
- Molan P. C., “Potential of honey in the treatment of wounds and burns,” American Journal of Clinical Dermatology, vol. 2, no. 1, pp. 13–19, 2001.View at: Google Scholar.
- Patton T., Barrett J., Brennan J., and Moran N., “Use of a spectrophotometric bioassay for determination of microbial sensitivity to manuka honey,” Journal of Microbiological Methods, vol. 64, no. 1, pp. 84–95, 2006.View at: Publisher Site | Google Scholar.
- Radwan S. S., El-Essawy A. A., and Sarhan M. M., “Experimental evidence for the occurrence in honey of specific substances active against microorganisms,” Zentralblatt fur Mikrobiologie, vol. 139, no. 4, pp. 249–255, 1984.View at: Google Scholar.
- Schepartz A. I. and Subers M. H., “The glucose oxidase of honey I. Purification and some general properties of the enzyme,” Biochimica et Biophysica Acta, vol. 85, no. 2, pp. 228–237, 1964.View at: Google Scholar.
- Sneath, P. H.; Mair, N. S.; Sharpe, M. F. and Holt, J. G. (1986). Bergeys Manual of Systematic Bacteriology. Williams and Wilkins. Baltimore.
- Tomas –Barberan ,F.A .; Viguer , C. and Ferreres,F.;Garcia-Lorente,F.(1993) Flavonoids in Honey of different geographical origion,ZLebensm unters Forsch,196:38-