##plugins.themes.academic_pro.article.main##

Abstract

The objective of this experiment was to examine the impact of using different levels of nanopropolis to drinking water on hematological parameters and heart, liver, gizzard, spleen and bursa gland relative weights of broiler chickens. A total of 144 one-day-old, unsexed broiler chickens with an average body weight of 40 g were used. The birds were distributed into 4 treatment groups (each with 36 birds), with three replicates in each group (12 birds per replicate). The first group drank reverse osmosis (R.O) drinking water without additions (control), whereas the second, third, and fourth groups drank reverse osmosis (R.O) drinking water containing 50, 100, and 150 µl of nano-propolis per liter, respectively. Higher some hematological variables such as RBC, Hb and PCV were observed in the 3rd and 4rd treatments compared to other treatments. Some blood parameters such as MCV, MCH, MCHC, heterophils, lymphocytes, heterophils/lymphocytes ratio and relative weight of heart, liver, gizzard, spleen and bursa gland were not affected by various amounts of nano-propolis. In conclusions, the addition of 100 and 150 μl of nanopropolis per liter of drinking water were improved some blood parameters that include RBC, Hb and PCV. Nano-propolis in current study without effect on MCV, MCH, MCHC, heterophils, lymphocytes, heterophils / lymphocytes ratio and relative weight of heart, liver, gizzard, spleen and bursa gland of broiler chickens.

Keywords

Nano-propolis Broiler Blood parameters

##plugins.themes.academic_pro.article.details##

How to Cite
Rasool H. Khalati, & Khalid C. K. Al-Salhie. (2023). Hematological Parameters and Relative Weight of some Organs for Broiler Chickens Supplemented by Different Levels of Nano Propolis. Texas Journal of Agriculture and Biological Sciences, 13, 115–121. Retrieved from https://zienjournals.com/index.php/tjabs/article/view/3451

References

  1. Krocko M, Canigova M, Bezekova J, Lavova M, Hascik P and Duckova V.2012. Effect of nutrition with Propolis and bee pollen supplements on bacteria colonization pattern in gastrointestinal tract of broiler chickens. Sci. Pap. Anim. Sci. Biotechnol, 45: 63–67.
  2. Klaric I, Pavic M, Miskulin I, Blazicevic V, Dumic A and Miskulin M.2018. Influence of Dietary Supplementation of Propolis and Bee Pollen on Liver Pathology in Broiler Chickens. Animals, 8(4): 54. https://doi.org/10.3390/ani8040054
  3. Tatli Seven P, Yilmaz S, Seven I, Cerci IH, Azman MA and Yilmaz M.2009. The effect of propolis on selected blood indicators and antioxidant enzyme activities in broilers under heat stress. Acta Veterinaria Brno, 78(1): 75-83. https://doi.org/10.2754/avb200978010075
  4. Capcarova M, Kolesarova A, Kalafova A, Galik B, Simko M, Juracek M and Toman R.2013. The role of dietary bee pollen in antioxidant potential in rats. Eurasian Journal of Veterinary Sciences, 29(3): 133–137. https://dergipark.org.tr/en/pub/eurasianjvetsci/issue/24806/262072
  5. Al-Ashoor DS and Al-Salhie KCK.2020. Effect of Adding Broccoli Leaves (Brassica oleracea L. var. italica) Extract to Drinking Water on Eggs Production and Intestinal Microflora of Japanese Quail Coturnix japonica Temmink & Schlegel, 1849. Basrah Journal of Agricultural Sciences, 33(2), 42–51. https://doi.org/10.37077/25200860.2020.33.2.04
  6. Al-Mosawy AMS and Al-Salhie KCK.2021.The Effect of Alcoholic Extract of Rhizomes of Greater Galangal (Alpinia galanga L.) on the Serum Antioxidant Enzymes for Japanese Quail During Oxidative Stress Induced by Hydrogen Peroxide. Basrah Journal of Agricultural Sciences, 34(1), 171–179. https://doi.org/10.37077/25200860.2021.34.1.15
  7. Mahjar NT and Al-Salhie KCK.2022. The effects of In Ovo Injection of Garlic (Allium sativum L.) Extract on Hatchability, Liver Enzymes and Antioxidant status of Broiler Chickens. Basrah Journal of Agricultural Sciences, 35(1), 61–70. https://doi.org/10.37077/25200860.2022.35.1.05
  8. Tekeli A, Kutlu, HR and Celik L.2011. Effects of Z. officinale and propolis extracts on the performance, carcass and some blood parameters of broiler chicks. Current Research in Poultry Science, 1(1): 12–23. https://doi.org/10.3923/crpsaj.2011.12.23
  9. Kacaniova M, Rovna K, Arpasova H, Cubon J, Hleba L, Pochop J, Kunova S and Hascik P.2012. In vitro and in vivo antimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens. Journal of Environmental Science and Health, Part A, Toxic/Hazardous Substances and Environmental Engineering , 47(11): 1665–1671. https://doi.org/10.1080/10934529.2012.687248
  10. Burdock GA.1998. Review of the biological properties and toxicity of bee propolis (Propolis). Food and Chemical Toxicology, 36:347–363.
  11. Troncarelli MZ, Brandao HM, Gern JC, Guimaraes AS and Langoni H.2013. Nanotechnology and antimicrobials in veterinary medicine. In: M_endez-Vilas A, editor. Microbial pathogens and strategies for combating them: science, technology and education. Badajoz: Formatex Research Center, Pp: 543–556.
  12. Brandao HM, Gern JC, Vicentini NM, Pereira MM and Andrade PVD.2011. Nanotecnologia: a pr_oxima revoluc¸~ao na agropecu_aria. Rev CRMV (Brasılia). 17:61–67.
  13. Afrouzan H, Amirinia C, Mirhadi SA, Ebadollahi A, Vaseji N and Tahmasbi G.2012. Evaluation of antimicrobial activity of propolis and nanopropolis against Staphylococcus aureus and Candida albicans. African Journal of Microbiology Research, 6(2): 421–425. https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.1032.1466&rep=rep1&type=pdf
  14. Sahlan M, Dienayati D, Hamdi D, Zahra S, Hermansyah H and Chulasiri M.2017. Encapsulation process of Propolis extract by casein micelle improves sunscreen activity. Makara Journal of Technology, 21(1):1–6. http://journal.ui.ac.id/technology/journal/article/view/3072
  15. Sies H.1991. Oxidative stress: from basic research to clinical application. The American Journal of Medicine, 91(3C): 31-38. https://doi.org/10.1016/0002-9343(91)90281-2
  16. Havsteen BH.2002. The biochemistry and medical significance of the flavonoids. Pharmacology and Therapeutics, 96(2-3): 67-202. https://doi.org/10.1016/s0163-7258(02)00298-x
  17. Hosnuter M, Gurel A, Babuccu O, Armutcu F, Kargi E and Isikdemir A.2004. The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury. Burns, 30: 121-125.
  18. Desmukh SD and Borle MN.1975. Studies on the insecticidal properties of indigenous plant products. Indian J. Enth. Pharm., 37 (1): 11-18.
  19. El-Ghaffar MAA and Hashem MS.2009. Immobilization of α‐amylase onto chitosan and its amino acid condensation adducts. Journal of Applied Polymer Science, 112 (2): 805-14.
  20. El-Ghaffar MAA and Hashem MS.2010. Chitosan and its amino acids condensation adducts as reactive natural polymer supports for cellulase immobilization. Carbohydrate Polymers, 81 (3): 507–516. http://doi.org/10.1016/j.carbpol. 2010. 02. 025.
  21. Campbell TW.1995. Avian hematology and cytology. Iowa State University Press, 176-198 p. https://bit.ly/3r2Emh6
  22. SPSS .2016. Statistical Packages of Social Sciences. IBM Corp. Released 2016. IBM SPSS Statistics for Windows, Version 24.0. Armonk, NY: IBM Corp. https:// www. ibm. com/ analytics/spss-statistics-software
  23. Duncan DB.1955. Multiple range and multiple F tests. Biometrics, 11: 1–42. https://doi.org/10.2307/3001478
  24. .Huang WY, Cai YZ and Zhang Y.2010. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr. Cancer, 62(1):1–20.
  25. Cui-ping Z, Shuai H, Wen-ting W, Shun P, Xiao-ge S, Ya-jing L and Fu-liang H.2014. Development of high-performance liquid chromatographic for quality and authenticity control of Chinese propolis. J. Food Sci., 79(7):C1315–22.
  26. Hassig A, Linag W, Schwabl H. and Stampfli K .1999. Flavonoids and tannins: plant-based antioxidants with vitamin character. Medical hypotheses, 52:479–481.
  27. Lee SW, Kim HJ, and Hwangbo S. 2001. Studies on the chemical characteristic of Korean propolis. Journal of Korean Society For Food Science of Animal Research, 21:383–388.
  28. Prytzyk E, Dantas AP, Salom˜ao K, Pereira AS, Bankova VS, De Castro SL, and Neto FRA.2003. Flavonoids and trypanocidal activity of Bulgarian propolis. Journal of Ethnopharmacology,88:189–193.
  29. Wang BJ, Lien YH, and Yu, ZR. 2004. Supercritical fluid extractive fractionation–study of the antioxidant activities of propolis. Food Chemistry, 86:237–243.
  30. Seven I, Aksu T, and Seven. PT.2010. The effects of propolis on biochemical parameters and activity of antioxidant enzymes in broilers exposed to lead-induced oxidative stress. Asian-Australasian Journal of Animal Sciences, 23:1482–1489.
  31. Bratter C, Tregel M, Liebenthal C and Volk H.1999. Prophylactic effectiveness of propolis for immunostimulation: a clinical pilot study. Forsch Komplementarmed, 6:256-260.
  32. Haro A, Aliaga IL, Francisco L, Barrionuevo M, Alfe’rez MJM and Campos MS.2000. Beneficial effect of pollen and/or propolis on the metabolism of iron, calcium, phosphorus, and magnesium in rats with nutritional ferropenic anemia. J. Agric. Food Chem., 48:5715–5722.
  33. Galal A, Abdel-Motaal AM, Ahmed AMH and Zaki TG. 2008. Productive performance and immune response of laying hens as affected by dietary Propolis supplementation. International Journal of Poultry Science, 7: 272–278.
  34. Omar REM, Mahmoud EM, Karusa MM, Randa AA. 2003. Effect of additives propolis and Nigella sativa seed oil on some behavioral patterns, performance products and blood parameters in Sasso chickens. Egyptian Poultry Science, 21:140–151.
  35. Attia YA, Abd Al-Hamid AE, Ibrahim, MS, Al-Harthi MA, Bovera F, Elnaggar A Sh. 2014. Productive performance, biochemical and hematological traits of broiler chickens supplemented with propolis, bee pollen, and mannan oligosaccharides continuously or intermittently. Livestock Science, 164:87–95.
  36. Abou-Zeid AE, El-Damarawy SZ, Mariey YA and El-Kasass SM.2015. Effect of dietary propolis supplementation on performance and activity of antioxidant enzymes in broiler chickens. Egyptian J. Nutrition and Feeds, 18(2) Special Issue: 391- 400.
  37. Abdel-Rahman MA and Mosaad GM. 2013. Effect of propolis as additive on some behavioral patterns, performance and blood parameters in Muscovy broiler ducks. Journal of Advanced Veterinary Research,3: 64–68.
  38. Torki M, Soltani J and Mohammadi H.2015. Effects of Adding Ethanol Extract of Propolis and Cumin Essential Oil to Diet on the Performance, Blood Parameters, Immune Response and Carcass Traits of Broiler Chicks. Iranian Journal of Applied Animal Science, 5(4): 911-918.
  39. Eyng C, Murakami AE, Santos TC, Silveira TGV, Pedroso RB, Lourenço DAL .2015. Immune Responses in Broiler Chicks Fed Propolis Extraction Residue-supplemented Diets. Asian-Australasian Journal of Animal Sciences, 28(1): 135-142. https://doi.org/10.5713/ajas.14.0066
  40. Sforcin JM .2007. Propolis and the immune system: a review. Journal of Ethnopharmacology, 113: 1–14.
  41. Denli M, Cankaya S, Silici S, Okan F and Uluocak AN.2005. Effect of dietary addition of Turkish propolis on the growth performance, carcass characteristics and serum vari¬ables of quail (Coturnix coturnix japonica). Asian Aust. J. Anim. Sci., 18: 848-854.
  42. Mahmoud UT, Amen OA, Applegate TJ and Cheng HW .2017. Brazilian Propolis Effects on Growth, Productivity Performance, Gut Characteristics and Physiological Changes in Broiler Chickens. International Journal of Poultry Science, 16: 169-179.