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Abstract
Twenty-two fungal isolates were isolated from five samples of oil-polluted water, the ability to grow on a solid mineral salt medium with 2% of crude oil was achieved; only seven isolates (WA2, WB3, WB7, WC2, WD3, WE2, and WE5) give clear-growing fungal colonies with different diameters. These fungal isolates are found to be more predominant in the polluted water samples, then they will be identified as Aspergillus fumigatus, Aspergillus niger, Trichoderma viridae, Fusarium verticelloides, penicillium oxysporum, Candida albicans, Aspergillus flavus respectively. In the present study, the primary and secondary screening of the ability of the isolates to degrade the crude oil was showed significant differences between the isolates on growth in the liquid medium containing 2% of crude oil and also in the percentage of crude oil biodegradation, while the isolate A. niger showed the highest ability compared with other studied isolates to consume the crude oil as carbon source with the best ratio of biodegradation reached to 95% after 28 days of incubation and the dry weight reached to 3.2 g after 7 days of incubation. Also, the results of GC showed the highest percentage loss of crude oil by A. niger which recorded 95% after 28 days of incubation, these results confirm the results of the screening tests for fungal isolates in terms of the similarity of the crude oil biodegradation rate after 7 days of incubation.
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References
- Arun, K.; Ashok, M. and Rajesh, S., Crude oil constitution, degradation pathway, and associated bioremediation microflora: an overview (2016) 1(7): 1420-1439.
- Xu, X.; Liu, W.; Tian, S.; Wang, W.; Qi, Q.; Jiang, P.; Gao, X.; Li, F.; Li, H.; Yu, H. Petroleum Hydrocarbon-Degrading Bacteria for the Remediation of Oil Pollution Under Aerobic Conditions: A Perspective Analysis. Front. Microbiol. (2018) 9, 2885. [CrossRef] [PubMed]
- Hewelke, E.; Szatyłowicz, J.; Hewelke, P.; Gnatowski, T.; Aghalarov, R. The Impact of Diesel Oil Pollution on the Hydrophobicity and CO2 Efflux of Forest Soils. Water Air Soil Pollut. (2019) 229, 1–11. [CrossRef] [PubMed]
- Oil Tanker Spill Statistics Published. Available online: https://www.itopf.org (accessed on 25 November 2020).
- Zouboulis, A.; Moussas, P. Groundwater and Soil Pollution: Bioremediation. In Encyclopedia of Environmental Health; Elsevier BV: Amsterdam, the Netherlands,( 2011) pp. 1037–1044.
- Kumar, L.; Bharadvaja, N. Enzymatic bioremediation: A smart tool to fight environmental pollutants. In Smart Bioremediation Technologies; Elsevier BV: Amsterdam, The Netherlands, (2019) pp. 99–118
- Atlas, R. M, Microbial degradation of petroleum hydrocarbons: an environmental perspective, Microbiology Review, 1981; 45(1): 108-209
- Hidayat, A. and Tachibana, S., Biodegradation of aliphatic hydrocarbon in three types of crude oil by Fusarium. sp. F092 under stress with artificial seawater. Journal of Environmental Science Technology, 2012; 5(1): 64-73
- Chaillan F., Fleche L.E.A., Bury E., Phantavong Y.H., Grimont P., Saliot A., and J. Oudot, Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms. Res. Mic
- Afzal, M.; Rehman, K.; Shabir, G.; Tahseen, R.; Ijaz, A.; Hashmat, A.J.; Brix, H. Large-scale remediation of oil-contaminated water using floating treatment wetlands. NPJ Clean Water 2019, 2, 3. [CrossRef]
- Lefebvre, O.; Habouzit, F.; Bru, V.; Delgenes, J.P.; Godon, J.J.; Moletta, R. Treatment of Hypersaline Industrial Wastewater by a Microbial Consortium in a Sequencing Batch Reactor. Environ. Technol. 2004, 25, 543–553. [CrossRef] [PubMed]
- Abdulrasheed, M.; Zakaria, N.N.; Roslee, A.F.A.; Shukor, M.Y.; Zulkharnain, A.; Napis, S.; Convey, P.; Alias, S.A.; Gonzalez-Rocha, G.; Ahmad, S.A. Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica. Antarct. Sci. 2020, 32, 1–13. [CrossRef]
- Edbeib, M.F.; Wahab, R.A.; Huyop, F. Halophiles: Biology, adaptation, and their role in decontamination of hypersaline environments. World J. Microbiol. Biotechnol. 2016, 32, 135. [CrossRef]
- Sarıkoç, S. Fuels of the Diesel-Gasoline Engines and Their Properties. In Diesel and Gasoline Engines; IntechOpen: London, UK, 2020; pp. 1–16.
- AI-Nasrawi, H., Biodegradation of crude oil by fungi isolated from Gulf of México. Journal of Bioremediation & Biodegradation, 2012; 3(4): 1-6
- Ameen F., Moslem M.A., Hadi S., and A. Al-Sabri, Biodegradation of cellulosic materials by marine fungi isolated from South Corniche of Jeddah, Saudi Arabia. J. Pure. Appl. Microbiol, 2014; 8: 3617-3626.
- Panˇci´c, M.; Köhler, E.; Paulsen, M.L.; Toxværd, K.; Lacroix, C.; Le Floch, S.; Hjorth, M.; Nielsen, T.G. Effects of oil spill response technologies on marine microorganisms in the high Arctic. Mar. Environ. Res. 2019, 151, 104785. [CrossRef] [PubMed]
- Mohammadi, L.; Rahdar, A.; Bazrafshan, E.; Dahmardeh, H.; Susan, A.B.H.; Kyzas, G.Z. Petroleum Hydrocarbon Removal from Wastewaters: A Review. Processes 2020, 8, 447. [CrossRef] 37.
- Varjani, S.; Upasani, V.N. A new look on factors affecting microbial degradation of petroleum hydrocarbon pollutants. Int. Biodeterior. Biodegrad. 2017, 120, 71–83. [CrossRef]
- Mittal, A. and Singh, P., Studies on biodegradation of crude oil by Aspergillus niger. The South Pacific Journal of Natural Science, 2014; 27(1): 27-60.
- Watanabe, T., Pictorial atlas of soil and used fungi: morphology and key to species. 2nd edition. India. CRC Press., 2002.
- Hari S., Isolation and screening of hydrocarbon-degrading fungi from soil. Department of Applied Microbiology and Biotechnology, 2003; 22: 112-115.
- Ijah, U. J. J. and Antai, S. P. (2005). Changes in physicochemical properties and fungal population after application of crude oil to the soil. Journal of Environmental Sciences 9:64-72.
- Ojo, O. A. (2006). Petroleum-hydrocarbon utilization by native bacterial population from a wastewater carnal Southwest Nigeria. African Journal of Biotechnology 5 (4) 333-337.
- Boyle, C. D. and 9.R Kropp. 1992. Development and comparison of methods for measuring growth of filamentous fungi on wood. Can. J. Microbiol., 38: 1053-1060.
- Olukunle, O. F., Boboye, B. and Ikuomola, O. T. (2012). Indigenous bacteria and fungi are responsible for the bioremediation of oil-polluted water in Ondo water State, Nigeria. Environtropica, 8, 138-148
- Akinde, S. B. and Obire, O. (2008). Aerobic fungi and petroleum utilizing- fungi from cow dung and poultry manure. World Journal of Microbiology and Biotechnology. 24, 1999-2002
- Mohsenzadeh, F.; Rad, C.A. and Akbari, M., Evaluation of oil removal efficiency and enzymatic activity in some fungal strain for bioremediation of petroleum – polluted soil. Iranian Journal of Environmental Health Science & Engineering. www.IJehse.com/Content/9/1/26. 2016.
- George- Okafor, U.; Tasie, F. and Okafor, F. M, Hydrocarbon degradation potentials of indigenous fungal isolates from petroleum contaminated soils. Journal of physical and Nature. Science, 2019; 3(1): 1-6.
- Mance a – Lopez, M. E.; Rodriguez, M. T.; Rios – Leal, E Esparza- Carcia, F.; ChavezGommez, B.; Rodriguez- Vazques, R. and Barrera- Cortes, J., Fungi and bacteria isolated from two highly polluted soils for hydrocarbon degradation. Journal of Acta Chimica Slovenica, 2014; 54: 201-209
- Damisa, D., Oyegoke, T. S., Ijah, U. J. J., Adabara, N. U., Bala, J. D. and Abdulsalam, R. (2013). Biodegradation of petroleum by fungi isolated from unpolluted Tropical soil. International journal of applied biology and pharmaceutical technology 4(2): 136-140.
- Dangi, A.K.; Sharma, B.; Hill, R.T.; Shukla, P. Bioremediation through microbes: Systems biology and metabolic engineering approach. Crit. Rev. Biotechnol. 2019, 39, 79–98. [CrossRef] [PubMed]
- Rv, X.M.R. Microorganisms Metabolism during Bioremediation of Oil Contaminated Soils. J. Bioremediation. Biodegrad. 2016, 7, 1–6. [CrossRef]
- Okoro, C.C. and Amund, O.O., Biodegradation of produced water hydrocarbons by Aspergillus fumigates. Journal of American Science, 2015; 6(3): 143-149.
- Davies, J. S. and Westlake, D., W, Crude oil utilization by fungi Journal of Candian Microbiology, 2010; 25(2): 146-156
- Levin L.A., and A. Forchiassin, Degradation of organic pollutants by the white-rot basidiomycete Trametes trogii. International Biodeterioration Biodegradation, 2017; 52: 15
- Elad, L., Chet, I. and Henis, Y. (2020). A Selective Medium for Improving Quantitative Isolation of Trichoderma sp. from Soil. Phytoparasitica 9(1): 59-67
- Varjani S.J., and V.N. Upasani, Biodegradation of petroleum hydrocarbons by the oleophilic strain of A. fumigatus NCIM 5514. Bioresource Technology, 2016; 222: 195-201