This article presents the results on the presence, activity, localization, properties and functional role of the carbonic anhydrase enzyme involved in the carbon metabolism of microalgae - euglena (which has a specific nutritional feature similar to heterotrophic and protozoa) Chlamydomonas and Dunaliella grown in intensive and mass culture, depending on the activity of various forms of carbonic anhydrase on the concentration of CO2 and nitrogen in the gas phase and medium. As analyzes have shown, all studied species and strains of algae have carbonic anhydrase activity, depending on the type of strain and cultivation conditions, 0,10-3,60 per mg of dry matter or 12-416 per chlorophyll unit. The lowest activity of the carbonic anhydrase enzyme was observed in the Dunalilla minute algae grown in an open-air semiproduction facility. In laboratory conditions grown in glass vessels, the minimum enzyme activity is observed in Chlamydomonas Reinhardii UA-5-16. The study of the localization of carbonic anhydrase in various cellular fractions of algae showed that in Dunaliella and Chlamydomonadal algae. The enzyme activity is mainly localized in the soluble fraction and gives similar values for both the homogeneous and the fraction of soluble proteins. In eugelena algae, a certain part (up to 40%) of carbonic anhydrase activity is localized in the membrane-bound fraction


Water grass Chlorella Growing water grass Enzymes


How to Cite
Bakhodirkhodja Ismailhodjaev, Karamat Kuatbekova, Bibiosiya Kholmirzaeva, Nasibov Boburbek, Jakhongirmirzo Mirzaqubulov, Nurali Eskaraev, & Nurjamal Abduraimova. (2022). Activity, patterns, and localization of carbonic acid enzymes in algae used in wastewater treatment. Texas Journal of Engineering and Technology, 14, 11–17. Retrieved from https://zienjournals.com/index.php/tjet/article/view/2704


  1. Kh.O.Berdykulov Pulsed light irradiation as a factor in the creation of productive forms of chlorella: Abstracts. (report 6th All-Union. conf. photovoltaics of plants. - Lviv. 1980). 117 p.
  2. A.M.Muzafarov and T.T.Taubaev Cultivation and application of microalgae.(Tashkent: Fan. 1984). 122 p.
  3. N.A.Pronina, S.Avramova, D.Georgiev, V.E.Semenenko Dynamics of carbonic anhydrase activity of Chlorella and Scenedesmus during cell adaptation to high intensity light and low CO2 concentration (Plant Physiology, Tashkent, 1981, Issue. I.) pp. 43-52.
  4. H.D.Hasic, F.A.Anigley, Saltindused dissociation of carbonicanhydrass from intact cells of Chlamydomonas Reinhardtii (Plant Physiol, 1990, No. 1.) pp.380-383.
  5. V.E.Semenenko, S.Avramova, D.Georgiev, N.A.Pronina Comparative study of the activity and localization of carbonic anhydrase in Chlorella and Scenedsmus cells, (Russ, Tashkent, 1977) pp.1055-1059.
  6. V.E.Semenenko, S.Avramova, D.Georgiev, N.A. Pronina On the light dependence of carbonic anhydrase activity in Chlorella and Scenedsmus cells, (Russ. Tashkent, 1979). pp.1069-1075.
  7. V.E.Semenenko, Biotechnology of photoautotrophic biosynthesis and the problem of industrialization of photosynthesis: state and development prospects: Abstracts of reports. (All-Union. conf. Industrial cultivation of microalgae, Moskov, 1985). Pp.92-95.
  8. N.A.Pronina, V.E.Semenenko Localization of membrane-bound and soluble forms of carbonic anhydrase in chlorella cells. (Plant Physiology, Tashkent,1984). Pp.241-251.
  9. Z.M.Ramazanov Adaptive changes and regulation of the synthesis of a soluble form of carbonic anhydrase in photosynthetic chlorella cells. (Abstract of thesis. Cand. dis., Moskov, 1984). 21 p.
  10. V.B.Borodin, E.D.Demidov The effect of carbonic anhydrase inhibitors on the Warburg effect and the release of glycolic acid by chlorella cells: Proceedings of the All-Union. (meeting: The kinetics of photosynthetic carbon metabolism in plants., Tallinn, Valgus. 1985) pp.99-103.
  11. S.Y.Yang, M.Tsuzukii, S.Miyachi Carbonic anhydrase of Chlamydomonas: Purification and studies on its induction using anti-Chlamydomonas carbonic anhydrase antiserum. (Ibid, 1985) pp.25-34.
  12. T. G.Williams, D.H.Hurpin The role of external carbonic anhydrase inorganic carbon acquisition by Chlamydomonasreinhardii at alkaline PH. (Plant Physiol, V.83., No.1. 1987). Pp. 92-96.
  13. D.A.Aliev, N.M.Guliev Carbonic anhydrous plants. (Moskov: Science, 1990). 175 p.
  14. K.Palmqvist, Z.M.Ramazonov, G.Samuelsson, The role of extracelluter carbonic anhydrase for accumulation of inorganic carbon in the algae Chlamydomonasreinhardii. A comparison between wildtype and cell-wall-less mutant cells (Physiol.Plant, 80.No.2. 1990) pp.267-276.
  15. Z.Ramasanov, K.Palngvist, G.Samuelsson, Organization of a CO2 - concentrating mechanism in green microalgae (Physiol. Plant, V.79., No 2., 1990.) pp. 2-6.
  16. M.G.Vladimirova, V.E.Semenenko Intensive culture of unicellular algae. (Academy of Sciences of the USSR. Moscov, 1962) 60 p.
  17. B.V.Gromov, A.A.Kondratyeva, K.A.Mamkeeva High-temperature strain Chlamydomonasreinhardii-449 under culture conditions (Bot. Zh. Tashkent, 58., No. I. 1973). Pp.127-132.
  18. V.E.Semenenko, A.A.Abdullaev Parametric control of β-carotene biosynthesis in cells under conditions of intensive culture (Plant Physiology: Tashkent, Issue. I. – 1980). Pp.31-37.
  19. Kh.A. Berdykulov Biological features of promising phototrophic microalgae and their methods of mass cultivation: (Author's abstract. doct. diss. Tashkent. 1991). 44 p.
  20. Z.M.Ramazanov, Chan-Van-Tya, V.E.Semenenko On the localization of carbonic anhydrase in the cells of the halophilic alga Dunaliellasalina (Abstracts. report USh conf. on spore plants of Central Asia and Kazakhstan. Tashkent, Fan. – 1989). Pp. 73-74.
  21. N.A.Pronina, Localization, properties and functional role of carbonic anhydrase in algal cells. (Abstract of thesis. Cand. dis., Moskov 1982) 22 p.
  22. D.Kerridge, K.Trypton Biochemical logic. (Moskov, Mir: 1974). 169 p.
  23. E.D.Demidov, E.A.Pavlova Carbonic anhydrase activity and Chlorellasp cell agent. K.KCO3 in the process of nitrogen starvation (Report, Academy of Sciences of the USSR. 313. No.5,1990). Pp.1270-1274.
  24. Ismailkhujaev, B., & Abdukodirova, M. (2020, July). Assessment of the effectiveness of biological treatment wastewater at “Binokor” aeration station located at Urta Chirchik district of Tashkent region. In IOP Conference Series: Materials Science and Engineering (Vol. 883, No. 1, p. 012082). IOP Publishing.